色谱

色谱 ›› 2011, Vol. 29 ›› Issue (02): 131-136.DOI: 10.3724/SP.J.1123.2011.00131

• 研究论文 • 上一篇    下一篇

离子液体对胶束电动色谱胶束微结构以及分离效果的影响

余美娟, 杭栋, 曹玉华*   

  1. 江南大学化学与材料工程学院, 江苏 无锡 214122
  • 收稿日期:2010-10-11 修回日期:2010-11-26 出版日期:2011-02-28 发布日期:2007-03-25
  • 通讯作者: 曹玉华,博士,教授,主要从事毛细管电泳工作
  • 基金资助:

    国家自然科学基金项目(No. 20775028).

Effects of ionic liquids on micellar microstructures and separation performance in micellar electrokinetic chromatography

YU Meijuan, HANG Dong, CAO Yuhua*余美娟, 杭栋, 曹玉华*   

  1. School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China
  • Received:2010-10-11 Revised:2010-11-26 Online:2011-02-28 Published:2007-03-25

PDF

526

被引次数

11

摘要： 考察了离子液体对胶束电动色谱胶束微结构以及分离效果的影响。研究结果表明,离子液体使胶束的表面电荷密度变小、粒径变大及其内核极性增大。以泼尼松、氢化可的松和泼尼松龙为分析对象,氢化可的松与泼尼松龙在十二烷基硫酸钠(SDS)胶束体系中不能实现分离,而在SDS-离子液体混合介质(20 mmol/L SDS-10 mmol/L 1-丁基-3-甲基咪唑四氟硼酸盐-50 mmol/L硼砂-硼酸缓冲液,pH 8.4)中,3种物质可在17 min内达到基线分离。各组分在2～100 mg/L范围内呈良好的线性关系,检出限(信噪比为3)分别为1.0、1.1和1.0 mg/L。应用本方法测定化妆品样品中上述3种皮质激素的含量及加标回收率,回收率为95.1%～117%。该方法样品处理简便,快速、准确、重复性好,可用于化妆品的质量监控。

关键词: 化妆品, 胶束电动色谱, 离子液体, 皮质激素, 微结构

Abstract: The effects of ionic liquids on micellar microstructures and separation performance in micellar electrokinetic chromatography (MEKC) were investigated. The experimental results showed that the addition of ionic liquids into micellar system would result in a decreased micellar surface charge density, an enlarged size of micelle and a slight enhancement of the polarity in the inner core of micelle. Prednisone, hydrocortisone and prednisolone were analyzed with MEKC to evaluate the separation performance. Hydrocortisone and prednisolone could not be separated in sodium lauryl sulfate (SDS) micellar system. However, the three analytes could be baseline separated in the mixed system of ionic liquids and SDS (20 mmol/L SDS-10 mmol/L 1-butyl-3-methyl imidazolium tetrafluoroborate-50 mmol/L borax, pH 8.4) within 17 min. Notably, the linearities of the three analytes ranged from 2 to 100 mg/L and the detection limits based on the ratio of signal to noise of 3 were 1.0, 1.1 and 1.0 mg/L for prednisone, hydrocortisone and prednisolone, respectively. The method has been used in the analysis of corticosteroids in cosmetic samples. The recoveries for the three analytes were between 95.1% and 117%. This method has the advantages of simple pretreatment, high accuracy, good reproducibility, and can be applied to the quality control of cosmetics.

Key words: corticosteroids, cosmetics, ionic liquids, micellar electrokinetic chromatography (MEKC), microstructure