色谱

色谱

• 研究简报 • 上一篇    下一篇

高效液相色谱法测定饮料类食品中的类雌激素

韩灏1,2,邵兵1,马亚鲁2,吴国华1,薛颖1   

  1. 1. Beijing Center for Disease Control and Prevention, Beijing 100013, China; 2. Department of Applied Chemistry, School of Science, Tianjin University, Tianjin 300072, China
  • 收稿日期:2004-03-19 修回日期:1900-01-01 出版日期:2005-03-30 发布日期:1988-12-25
  • 通讯作者: 邵兵
  • 基金资助:

Determination of Estrogen-Like Compounds in Beverages by High Performance Liquid Chromatography

HAN Hao1,2, SHAO Bing1, MA Yalu2, WU Guohua1, XUE Ying1   

  1. 1. Beijing Center for Disease Control and Prevention, Beijing 100013, China; 2. Department of Applied Chemistry, School of Science, Tianjin University, Tianjin 300072, China
  • Received:2004-03-19 Revised:1900-01-01 Online:2005-03-30 Published:1988-12-25

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摘要： 利用OASIS HLB固相萃取柱富集和净化样品,采用高效液相色谱分离,紫外及荧光检测,建立了不同饮料基质中的壬基酚(NP)、辛基酚(OP)和双酚A(BPA)等类雌激素的测定方法,并用该方法对市售部分产品进行了测定。不同加标水平的BPA,NP和OP的回收率为91.08%~103.19%,其相对标准偏差为0.5%~5.49%。该方法具有操作简单、灵敏度高和重现性好等优点。

关键词: 高效液相色谱法, 固相萃取, 壬基酚, 双酚A, 辛基酚, 饮料

Abstract: A comprehensive analytical method based on high performance liquid chromatography-ultravioiet-fluorescence detection (HPLC-UV-FL) has been established for the determination of bisphenol A (BPA), nonylphenol (NP) and octylphenol (OP) in beverages with different samples and concentrations. Clean-up of samples was performed on an OASIS HLB solid extraction cartridges (6 mL, 200 mg). The method was successfully applied to the investigation of residual bisphenol A, nonylphenol and octylphenol in the beverages (mineral waters and soft drinks) available in commercial markets in Beijing. Recoveries for bisphenol A, nonylphenol and octylphenol at different spiked levels were ranged from 91.08% to 103.19% with relative standard deviations of 0.5% to 5.49%. The method is simple with good reproducibility and selectivity, and high sensitivity.

Key words: beverages , bisphenol A, high performance liquid chromatography, nonylphenol, octylphenol, solid phase extraction