色谱

色谱

• 技术与应用 • 上一篇    

固相萃取-高效液相色谱法测定钙强化食品中的维生素D

赵榕,薛颖,吴国华,赵海燕,罗仁才

  

  1. Beijing Center for Disease Prevention and Control, Beijing 100013, China

  • 收稿日期:2007-06-22 修回日期:2007-09-27 出版日期:2008-01-30 发布日期:1984-09-25
  • 通讯作者: 赵榕

Determination of vitamin D in calcium fortified foods using
solid-phase extraction-high performance liquid chromatography

ZHAO Rong, XUE Ying, WU Guohua, ZHAO Haiyan, LUO Rencai

  

  1. Beijing Center for Disease Prevention and Control, Beijing 100013, China

  • Received:2007-06-22 Revised:2007-09-27 Online:2008-01-30 Published:1984-09-25
  • Contact: Zhao Rong

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摘要：

以含有体积分数为20%的0.95 mol/L柠檬酸水溶液的二甲基亚砜作为维生素D的破壁溶液,利用Chromabond XTR固相萃取柱(14500 mg, 70 mL)对样品进行提取和净化,建立了测定钙强化食品中维生素D的固相萃取-高效液相色谱方法。方法的线性范围为0.1～100.0 μg/mL,线性相关系数为0.999。方法的定性检出限为0.01 μg/g,定量检出限为0.03 μg/g。低(0.1 μg/g)、中(0.5 μg/g)、高(1.0 μg/g)三个浓度水平的加标回收率分别为106.2%,99.5%和100.1%,相对标准偏差小于10%。

关键词: 钙强化食品 , 高效液相色谱法, 固相萃取, 维生素D

Abstract:

A method for the determination of vitamin D in calcium fortified foods using solid-phase extraction-high performance liquid chromatography has been developed. The Chromabond XTR solid-phase extraction column (14500 mg, 70 mL) was used to extract and clean-up the sample. The calibration curve of vitamin D showed good linearity in the range of 0.1-100.0 μg/mL with correlation coefficient of 0.999. The limit of qualification was 0.01 μg/g and the limit of quantification was 0.03 μg/g. The average recoveries at three spiking levels were 106.2%, 99.5%, 100.1%, and the relative standard deviations were lower than 10%.

Key words: calcium fortified foods
, solid-phase extraction (SPE), vitamin D, high performance liquid chromatography (HPLC)