| 丝素纤维/硫酸钙抗感染骨材料的制备及性能 |
| The preparation and properties of Silk Nanofibers/Calcium Sulfate composite on infective bone repair |
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| 摘要: 本研究通过控制丝素蛋白自组装过程制备了溶液状态下的丝素纳米纤维(silk fibroin nanofibers，SFFs)，与硫酸钙、万古霉素(Vancomycin，VCM)复合，制备了VCM/CS/SFFs抗菌骨材料。通过SEM、XRD、紫外分光光度计、万能力学试验机、抑菌圈、MTT等手段分别研究了复合材料的微观形貌与结构、药物释放、力学、抑菌及细胞相容性等性能。结果显示，与水作为固化液相比，随着SFFs溶液(0.0175mg?mL-1~2.1mg?mL-1)的加入，复合材料凝固时间可控，降解率逐渐降低、抗水性增强，韧性提高；同时随丝素纳米纤维含量的增加骨材料抗压强度表现为先增加后减小的趋势，一周内药物释放速率降低；材料同时具有抑菌作用；MTT实验结果显示，加入丝素纳米纤维后与纯的硫酸钙相比MC3T3细胞增殖明显。这一初期的研究结果将对改善α-半水硫酸钙的骨科临床使用提供一定的理论参考。 |
| 关键词: 关 键 词:丝素纳米纤维 半水硫酸钙 感染性骨缺损 力学性能 细胞相容性 |
| 基金项目: 国家自然科学基金青年科学基金资助项目(51502192,51503140,11502158);山西省基础研究计划项一般青年基金资助项目(2014021039-6)；山西省高等学校科技创新资助项目(2016142)；太原理工大学校青年基金资助项目(2014TD066,2013Z020)；太原理工大学引进人才资助项目(tyut-rc201270a);太原理工大学技术服务资助项目(143230043-J);山西省研究生教育创新项目(2017SY023) |
| Abstract: In the present study, silk fibroin nanofibers solution (SFFs) was processed through controlling silk protein molecules self-assembly process, which were incorporated with vancomycin (VCM)to calcium Sulfate (CS) for infective calcium sulfate-based materials. In this study, surface morphology of the material was observed using SEM. Structure of the materials was studied with XRD. Drug release property of materials was evaluated using UV absorbance. Mechanical property of the materials was tested using computer-controlled electronic universal testing machine. Antibactrial property was illustrated by inhibition zone. Cytocompatibility was assessed by MTT assay. The results showed that the addition of SFFs can not only effectively reduce the degradation rate of the composite material, but also enhance toughness and water resistance comparing to water as setting liquid. And the compressive strength first increased, then decreased as the concentration of SFFs from 0.0175mg?mL-1 to 2.1mg?mL-1. The results also showed that the composite with controllable setting time had excellent antibacterial properties and drug slow-release performance in a week as well as better cell proliferation with MC-3T3. The research suggested that incorporating silk fibroin nanofibers to calcium sulfate could develop wider clinical application of calcium sulfate to treat bone defects as academic reference. |
| Keywords: silk fibroin nanofibers calcium sulfate hemihydrate infective bone defect mechanical properties cytocompatibility |
| 投稿时间：2017-09-30 修订日期：2017-11-30 |
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