Structural Characterization and Spectroscopic Analysis of the Aloin
XIE Yun-fei1, HUAN Nan1, CAO Yuan-yuan1, WANG He-ya1, ZHONG Ying2, YAO Wei-rong1, QIAN He1*
1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China 2. Aloe Industry Committee of CAPNSE, China Rural Technology Development Center, Beijing 100045, China
Abstract：Aloe is widely used in various fields for its rich polysaccharides, proteins, amino acids, vitamins, active enzymes and trace beneficial elements to human body. However, the main active ingredient aloin is also an allergenic ingredient, which even may cause a severe allergic reaction. In this study, infrared spectroscopy, Raman spectroscopy applied to the structural characterization of the aloin. Density functional theory (DFT) is applied to the theoretical calculations using the B3LYP/6-31G (d) basis set vibration, which was helpful to understand the aloin molecular vibrational frequency. By comparing we choose the optimal experimental condition for water as solvent under alkaline conditions, the detection limit of the Aloin can reach a level of 5 ppm, which can be considered the theoretical basis for rapid detection of aloin content.
[1] Eshun K, He Q. Critical Reviews in Food Science and Nutrition, 2004, 44(2): 91. [2] Wamer W G, Vath P, Falvey D E. Free Radical Biology and Medicine, 2003, 34(2): 233. [3] ElSohly M A, Gul W, Murphy T P. International Immunopharmacology, 2004, 4(14): 1739. [4] Zonta F, Bogoni P, Masotti P, et al. Journal of Chromatography A, 1995, 718(1): 99. [5] Chiang H M, Lin Y T, Hsiao P L, et al. Journal of Food and Drug Analysis, 2012, 20(3): 646. [6] Nagaraju M, Ramulla S, Murthy N Y S. Asian Journal of Chemistry, 2011, 23(6): 2421. [7] Ramirez Duron R, Ceniceros Almaguer L, Cavazos Rocha N C, et al. Journal of AOAC International, 2008, 91(6): 1265. [8] Wang P G, Zhou W, Wamer W G, et al. Analytical Methods, 2012, 4(11): 3612. [9] Chen W, Zhang Y, Luo H, et al. Str. Pharm. J., 2004, 16: 57. [10] Nie S, Emory S R. Science, 1997, 275(5303): 1102. [11] Li J F, Huang Y F, Ding Y, et al. Nature, 2010, 464(7287): 392. [12] Ding L P, Fang Y. Applied Surface Science, 2007, 253(9): 4450. [13] Han Y M, Lin H, Chen Y Y. Studies and Technology, 2009, (2): 28. [14] Meng Y, Yan B Z, Hu G F. Journal of Beijing University of Chemical Technology, 2004, 31(3): 70. [15] Siesler H W, Ozaki Y, Kawata S, et al. Near-Infrared Spectroscopy: Principles, Instruments, Applications. Wiley-Vch, 2008.
