Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy
WANG Wei-ping1, 2, CHAI A-li2*, SHI Yan-xia2, XIE Xue-wen2, LI Bao-ju2*
1. College of Plant Protection, Shenyang Agricultural University, Shenyang 100866, China 2. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract：Clubroot, caused by Plasmodiophora brassicae, is considered the most devastating soilborne disease in Brassica crops. It has emerged as a serious disease threatening the cruciferous crop production industry in China. Nowadays, the detection techniques for P. brassicae are laborious, time-consuming and low sensitivity. Rapid and effective detection methods are needed. The objective of this study is to develop a Fourier transform infrared spectrometer (FTIR) technique for detection of P. brassicae effectively and accurately. FTIR and Real-time PCR techniques were applied in quantitative detection of P. brassicae. Chinese cabbages were inoculated with P. brassicae. By analyzing the FTIR spectra of P. brassicae, infected clubroots and healthy roots, three specific bands 1 105, 1 145 and 1 228 cm-1 were selected. According to the correlation between the peak areas at these sensitive bands and Real-time PCR Ct value, quantitative evaluation model of P. brassicae was established based on FTIR y=34.17+12.24x1-9.81x2-6.05x3, r=0.98 (p<0.05). To validate accuracy of the model, 10 clubroot samples were selected randomly from field, and detected by FTIR spectrum model, the results showed that the average error is 1.60%. This demonstrated that the FTIR technology is an available one for the quantitative detection of P. brassicae in clubroot, and it provides a new method for quantitative and quickly detection of Chinese cabbage clubroot.
王惟萍1, 2，柴阿丽2*，石延霞2，谢学文2，李宝聚2* . 基于傅里叶变换红外光谱的大白菜根肿病定量测评 [J]. 光谱学与光谱分析, 2015, 35(05): 1243-1247.
WANG Wei-ping1, 2, CHAI A-li2*, SHI Yan-xia2, XIE Xue-wen2, LI Bao-ju2* . Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(05): 1243-1247.
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