色谱

色谱 ›› 2013, Vol. 31 ›› Issue (7): 714-717.DOI: 10.3724/SP.J.1123.2012.12040

• 技术与应用 • 上一篇    

硼酸亲和整体柱在富集中药活性物质中的应用

胡青红1, 王超然1, 李静1,2, 王彦1, 谷雪1, 阎超1   

  1. 1. 上海交通大学药学院, 上海 200240;
    2. 上海通微分析技术有限公司, 上海 201203
  • 收稿日期:2012-12-25 修回日期:2013-01-22 出版日期:2013-07-28 发布日期:2013-07-17
  • 通讯作者: 阎超
  • 基金资助:

    国家自然科学基金项目(21175092,21105064);国家重大科学仪器设备开发专项(2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210);上海市自然科学基金项目(12ZR1413600).

Enrichment of cis-diols active components in traditional Chinese medicines by boronate affinity monolithic column

HU Qinghong1, WANG Chaoran1, LI Jing1,2, WANG Yan1, GU Xue1, YAN Chao1   

  1. 1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China;
    2. Unimicro (Shanghai) Technologies Co., Ltd., Shanghai 201203, China
  • Received:2012-12-25 Revised:2013-01-22 Online:2013-07-28 Published:2013-07-17

PDF

214

被引次数

6

摘要：

以4-乙烯基苯硼酸(VPBA)为功能单体,季戊四醇三丙烯酸酯(PETA)为交联剂,乙二醇和二甘醇为二元致孔剂,通过热引发自由基聚合反应,在毛细管内原位聚合制备得到一种poly(VPBA-co-PETA)毛细管整体柱。所制备的整体柱具有典型的硼酸亲和特性,可以特异性地捕获含有顺二羟基的化合物。在前期实验的基础上,将其用于两种中草药 蒲公英和刺果卫矛中含顺二羟基的小分子活性物质的富集。这两种中药提取物直接用高效液相色谱进行检测时,其活性成分绿原酸等因含量太低而使检测困难或无法检测;经过硼酸亲和整体柱富集后收集洗脱液进行检测,色谱峰响应则显著提高。该结果表明所制备的poly(VPBA-co-PETA)整体柱可以作为富集中药提取物中含顺二羟基活性物质的有效手段。

关键词: 富集, 活性物质, 硼酸亲和, 顺二羟基化合物, 整体柱, 中药

Abstract:

A novel poly(4-vinylphenylboronic acid (VPBA)-co-pentaerythritol triacrylate (PETA)) monolithic column was prepared by thermal-initiated copolymerization of VPBA and PETA in a binary porogen system comprising diethylene glycol and ethylene glycol. The fabricated monolithic column demonstrated good boronate affinity property, which can specifically capture cis-diols containing compounds. We developed such a monolithic column and applied it to enrich active components in Herba Taraxaci and Euonymus acanthocarpus. The extracts of the two traditional Chinese medicines were separated and enriched on μ HPLC by the prepared monolith, and the fractions were collected and examined by HPLC. The results showed that the responses of the corresponding peaks were greatly improved, indicating that the poly(VPBA-co-PETA) monolithic column can be efficiently used to enrich active components in traditional Chinese medicines containing cis-diols group.

Key words: cis-diols compounds, active components, boronate affinity, enrichment, monolithic column, traditional Chinese medicine (TCM)

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