| 高稳定性黑米碳点的绿色合成及其细胞成像应用 |
| Green synthesis of high stability black rice carbon dots and its application in cell imaging |
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| 摘要: 本文以绿色廉价的黑米为碳源,采用简单的水热法一步合成了碳点(Carbon dots,CDs)。所合成的碳点不仅对pH/NaCl溶液或温度变化不敏感,并且对多种金属离子具有免疫力,表现出优异的稳定性。同时,CDs在较短波长范围(410 nm至470 nm)表现出激发不依赖的发射行为,在较长波长范围(470 nm至510 nm)又表现出弱激发依赖的发射行为。由于其优异的荧光特性、稳定性和低毒性,我们将其应用于人口腔角质形成细胞(Hok)、宫颈癌细胞(Hela)和骨髓间充质干细胞(MC3T3)的体外成像,揭示了CDs有望作为杰出的细胞成像剂应用于生物医学领域的潜力。 |
| 关键词: 黑米碳点，荧光性质，稳定性，细胞成像 |
| 基金项目: |
| Abstract: In this work, carbon dots (CDs) were synthesized by a facile hydrothermal method using cheap black rice as a carbon source. The synthesized CDs exhibited excellent stability, which were not sensitive to changes in temperature or solution of pH/NaCl, and were immune to many metal ions. The purified CDs also had low cytotoxicity against human oral keratinocytes (Hok), cancer cells (cervical cancer, Hela), and bone marrow mesenchymal stem (BMSC, MC3T3) cells. Meanwhile, it behaved with excitation-independent behavior in the shorter wavelength range (410 nm to 470 nm) and excitation-dependent behavior in the longer wavelength range (470 nm to 510 nm). Due to the excellent fluorescence properties and stability, we applied them to the intracellular imaging of Hok, Hela, and MC3T3 cells, revealing the clinical potential of the CDs as an outstanding cell imaging agent. |
| Keywords: Black rice carbon dots fluorescence properties stability cell imaging |
| 投稿时间：2022-09-21 修订日期：2023-03-16 |
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