| 气相色谱-串联质谱法测定不同产地大米中的角鲨烯 |
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| 中文摘要:通过比较大米中角鲨烯的不同提取方法，选用优化后的索氏提取/气相色谱-串联质谱（GC-MS/MS）法对不同产地大米中的角鲨烯进行定性及定量分析。结果表明，该方法在1~50 mg/L质量浓度范围内线性良好,相关系数为0.998 7；检出限为0.1 mg/kg；在5、20 mg/kg 2个加标水平下的回收率可达96%~104%；相对标准偏差（RSD）不大于3.6%。对不同产地大米中角鲨烯的分析表明，该方法的灵敏度高、重复性好，可作为区分大米不同种属、产地和品质的有效方法。 |
| 中文关键词:气相色谱-质谱 角鲨烯 大米 索氏提取 |
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| Determination of Squalene in Rices from Different Areas by Gas Chromatography-Tandem Mass Spectrometry |
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| Abstract:Different extraction methods of bioactive component squalene in rices were studied and a new method was developed for the determination of squalene in rices.The squalene in rices from different areas were extracted by Soxhlet extract method,and analyzed by gas chromatography-tandem mass spectrometry（GC-MS/MS）.The results showed that the calibration curve was linear over the concentration of squalene in the range of 1-50 mg/L with a correlation coefficient of 0.998 7.The detection limit（LOD） was 0.1 mg/kg.The recoveries of squalene at two spiked levels of 5 mg/kg and 20 mg/kg were in the range of 96%-104% with relative standard deviations（RSDs） no more than 3.6%.The method was sensitive and reproducible,and could be used to distinguish effectively rices with different species,origins and qualities. |
| Key Words:gas chromatography-tandem mass spectrometry（GC-MS/MS） squalene rice Soxhlet extract method |
| 引用本文：李红，田福林，刘成雁，程光荣.气相色谱-串联质谱法测定不同产地大米中的角鲨烯[J].分析测试学报,2011,30(10):1179-1182. |
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